Peptide Reconstitution Calculator
Follow 4 simple steps to calculate exactly how many units to draw on your syringe. No math needed.
Choose Your Syringe
Which insulin syringe do you have? Most people use 0.5 ml or 1.0 ml.
Peptide Vial Amount
How many milligrams are in your vial? Check the label.
Bacteriostatic Water
How much water will you add? 2 ml is the most common.
Concentration: 2,500 mcg/ml
Desired Dose Per Injection
How much peptide per injection? Pick your unit below.
For research purposes only. Always verify with your healthcare provider.
For research purposes only. Always verify with your healthcare provider.
What Is Peptide Reconstitution?
Peptide reconstitution is mixing a freeze-dried (lyophilized) peptide powder with bacteriostatic water so it can be measured and injected. Most research peptides ship as dry powder in a sealed vial. You add a precise amount of bacteriostatic water to dissolve the powder before use.
The amount of water you add determines the concentration of your solution, which in turn determines how many units to draw on your syringe for each dose. This calculator does that math for you.
How to Reconstitute Peptides Step by Step
- 1.Clean the rubber stopper of your peptide vial with an alcohol swab and let it dry.
- 2.Using a syringe, draw the calculated amount of bacteriostatic water.
- 3.Insert the needle through the stopper and inject the water slowly down the inside wall of the vial. Do not aim directly at the powder.
- 4.Gently swirl the vial until the powder dissolves completely. Never shake it, as this can degrade the peptide.
- 5.Store the reconstituted vial in your refrigerator (2-8 degrees C). Use within 3 to 4 weeks.
Frequently Asked Questions
What is bacteriostatic water?
Bacteriostatic water is sterile water that contains 0.9% benzyl alcohol, which prevents bacteria from growing. It is the standard diluent for reconstituting peptides. Do not use regular sterile water, as your reconstituted peptide will degrade faster without the preservative.
How much bacteriostatic water should I add?
The amount depends on your vial size and desired concentration. Common amounts are 1 ml or 2 ml. Using more water makes each unit on your syringe contain less peptide, which can make it easier to measure small doses accurately. Our calculator handles this math for you.
How long does reconstituted peptide last?
When stored in the refrigerator (2-8 degrees C), reconstituted peptides with bacteriostatic water typically last 3 to 4 weeks. Never freeze reconstituted peptides, and always keep them away from light and heat.
What does 'units' mean on an insulin syringe?
On a standard U-100 insulin syringe, 1 unit equals 0.01 ml. So 10 units = 0.1 ml, and 100 units = 1 ml. A 0.5 ml syringe has 50 units, and a 0.3 ml syringe has 30 units. Our calculator converts your desired dose into syringe units so you know exactly how far to draw.
Which syringe size should I choose?
For most peptide doses, a 0.5 ml (50 unit) or 1.0 ml (100 unit) insulin syringe works well. A 0.3 ml syringe is great for very small doses because the markings are more spread out, making it easier to measure precisely. Choose the smallest syringe that still fits your dose volume.
Can I use the same syringe for reconstitution and injection?
It is best practice to use a larger syringe (like a 3 ml syringe with a 21-gauge needle) for reconstitution and a separate insulin syringe (29-31 gauge) for injection. The larger needle makes it easier to draw and inject bacteriostatic water into the vial.
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