Peptide Reconstitution Calculator
Calculate exactly how much bacteriostatic water to add and how many units to draw for your dose.
Amount printed on your vial (e.g., 5mg BPC-157)
Typically 1 to 2 ml. More water = lower concentration per unit.
e.g., 250 mcg for BPC-157 standard dose
What is Peptide Reconstitution?
Peptide reconstitution is the process of mixing a freeze-dried (lyophilized) peptide powder with a liquid so it can be measured and injected. Most research peptides arrive as a dry powder in a sealed vial, and you need to add bacteriostatic water to dissolve them before use.
Getting the reconstitution right is important because the amount of water you add directly determines how concentrated your solution is, which in turn determines how many units you need to draw on your syringe for a given dose.
How to Reconstitute Peptides Step by Step
- 1.Clean the rubber stopper of your peptide vial with an alcohol swab and let it dry.
- 2.Using a syringe, draw the calculated amount of bacteriostatic water.
- 3.Insert the needle through the stopper and inject the water slowly down the inside wall of the vial. Do not aim directly at the powder.
- 4.Gently swirl the vial until the powder dissolves completely. Never shake it, as this can degrade the peptide.
- 5.Store the reconstituted vial in your refrigerator (2-8 degrees C). Use within 3 to 4 weeks.
Frequently Asked Questions
What is bacteriostatic water?
Bacteriostatic water is sterile water that contains 0.9% benzyl alcohol, which prevents bacteria from growing. It is the standard diluent for reconstituting peptides. Do not use regular sterile water, as your reconstituted peptide will degrade faster without the preservative.
How much bacteriostatic water should I add?
The amount depends on your vial size and desired concentration. Common amounts are 1 ml or 2 ml. Using more water makes each unit on your syringe contain less peptide, which can make it easier to measure small doses accurately. Our calculator handles this math for you.
How long does reconstituted peptide last?
When stored in the refrigerator (2-8 degrees C), reconstituted peptides with bacteriostatic water typically last 3 to 4 weeks. Never freeze reconstituted peptides, and always keep them away from light and heat.
What does 'units' mean on an insulin syringe?
On a standard U-100 insulin syringe, 1 unit equals 0.01 ml. So 10 units = 0.1 ml, and 100 units = 1 ml. Our calculator converts your desired dose into syringe units so you know exactly how far to draw.
Can I use the same syringe for reconstitution and injection?
It is best practice to use a larger syringe (like a 3 ml syringe with a 21-gauge needle) for reconstitution and a separate insulin syringe (29-31 gauge) for injection. The larger needle makes it easier to draw and inject bacteriostatic water into the vial.
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